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. 2016 Dec 8;7:13823. doi: 10.1038/ncomms13823

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Copyright © 2016, The Author(s)

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(a) Kaplan-Meier plots of a survival experiment of drosophila flies orally inoculated with PAO1 or ΔDS did not show significant differences in survival between both strains (Mantel-Cox test; P=0.9191). The graph corresponds to a single representative experiment of a total of four independent experiments done (each using 50 flies per condition). (b) Kaplan-Meier plots of a survival experiment of drosophila flies inoculated with PAO1 or ΔDS showed a significant attenuation of ΔDS (Mantel-Cox test; P<0.0001, three independent experiments, each using 15 flies per condition: total 45 flies for each strain). (c) A survival experiment as the one shown in b but comparing seven transposon mutants including PA2077 and six well-established virulence factors of P. aeruginosa: ExoA, exotoxin; PhospC, phospholipase C; ExoS, exoenzyme S; LasR, quorum sensing transcriptional regulator; PilA, type 4 fimbrial precursor and FliM, flagellar motor switch protein. PA2077 and the rest of Tn mutants were not significantly different between them, but they all were significantly different from PAO1 (Mantel-Cox test, P<0.0001, 15 flies per strain). (d) LC/MS/MS spectrometry analysis homogenates of flies infected with PAO1 or ΔDS. The reconstructed ion chromatograms show detection of 10-HOME (m/z 155.1, left graph) and 7,10-DiHOME (m/z 141, right graph) in flies infected with PAO1 (shown in red), but not in those inoculated with ΔDS (shown in blue). (e) Graph of an infection experiment done in lettuce. The percent of established infections over the total number of inoculation events is graphed. Means were significantly different (ordinary one-way ANOVA, **, P<0.01, three independent experiments, error bars are s.d.). N, total of inoculation events; L, number of independent leaves inoculated.