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. 2016 Dec 9;7:13644. doi: 10.1038/ncomms13644

Figure 3. Polyubiquitinated HAUSP regulates global H3K56Ac and interacts with CBP and components of the Mediator/elongation complex.

Figure 3

(a) Knockdown of HAUSP in MDA-MB-231 and SAS cells decreased the global levels of H3K56Ac by Western blot analysis. (b) Co-immunoprecipitation assays showed the increased interaction between HAUSP and CBP under hypoxia in PC3 cells. (c) Knockdown of HAUSP in MDA-MB-231 cells decreased the interaction between HIF-1α and CBP by co-immunoprecipitation assays. (d) Co-immunoprecipitation assays showed that the K443R HAUSP mutant had a weaker interaction with HIF-1α and CBP under hypoxia compared to wild-type HAUSP when the indicated proteins were expressed in 293T cells. (e) Co-immunoprecipitation assays showed the interaction between HAUSP and the components of the Mediator complex in MDA-MB-231 cells. (f) Increased interaction between HAUSP and AFF4 in PC3 cells under hypoxia by co-immunoprecipitation assays. (g) The HAUSPK443R mutant had a decreased interaction with AFF4 compared to the wild-type HAUSP by co-immunoprecipitation assays when HAUSP (wild type or mutant) was overexpressed in 293T cells to pull down AFF4.