Skip to main content
. 2016 Dec 9;7:13644. doi: 10.1038/ncomms13644

Figure 6. Analysis of ChIP-seq results with identification of two motifs and confirmation of various factors binding to the KDM3A promoter regions.

Figure 6

(a) The numbers of overlapping set of genes that were bound by HAUSP and HIF-1α by ChIP-seq analysis. (b) GO analysis of the 31 genes that showed binding by both HAUSP and HIF-1α. (c) Identification of two consensus motifs that were bound by HAUSP and HIF-1α. (d) Gene track analysis of a representative gene, KDM3A, that contained motif-1 and motif-2 in its promoter regions. (e) The left panel shows the schematic diagram of the KDM3A promoter regions containing motif-1 that were checked by qChIP assays. qChIP analysis confirmed the binding of various factors to motif-1 in the KDM3A promoter regions. (f) The left panel shows the schematic diagram of the KDM3A promoter regions containing motif-2 that were checked by qChIP assays. qChIP analysis confirmed the binding of various factors to motif-2 in the KDM3A promoter regions. N, normoxia; H, hypoxia. Data from three independent experiments are expressed as mean±s.d. The asterisk (*) indicated statistical significance (P<0.05) between normoxia and hypoxia conditions, t-test.