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. 2016 Dec 14;6:38987. doi: 10.1038/srep38987

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Luminescence was induced in oral mucosal epithelial cell sheets derived from luc tg rats by adding luciferin. The observed luminescence correlates with the ATP levels. Scale bars represent 100 μm. (B) Time scale for ATP measurements using OEC sheets derived from luc tg rats. ATP levels of the cell sheet were measured at day 0 (D0, before preservation), day 7 ((D7) after preservation), and at 7 days after re-culturing (Re-D7). (C,D) Screening of basal medium using OEC sheets derived from luc tg rats. ATP levels at (C) day 7 after preservation and (D) re-culturing were calculated as the ration (%) of the ATP levels measured before preservation. *P < 0.05, **P < 0.001 (versus HBSS). Data are presented as mean ± SD of three independent experiments. (E) Screening of HBSS + additives using OEC sheets derived from luc tg rats. ATP levels at day 7 after preservation were calculated as the ration (%) of the ATP levels measured before preservation. *P < 0.05, **P < 0.001 (versus HBSS). Data are presented as the mean ± SD of three independent experiments.