Figure 1. The effect of TNFα on epoxygenated fatty acid levels.
HRMEC were treated with vehicle or TNFα (1 ng/ml), arachidonic acid and docosahexaenoic acid substrates were provided, and the level of their epoxygenated products was measured in the conditioned media by LC-MS/MS. These data are normalized to the total protein of the cell lysates. Each bar represents the mean ± SEM (n = 8 for EET measurements; n = 9 for EDP measurements).