Figure 1. Genomic Structures of Sp5 and the plaque formation and lysogenization capacities of mutant Sp5 phages.

(A) The genomic organizations of lambda phage and Sp5, the Stx2 phage of EHEC O157:H7 strain Sakai. Early and late regions are shown separately. Genes of known or reliably predicted functions³²,⁵⁶ are colored according to the functional category shown in the inset. The open arrows in Sp5 are the genes of unknown function that were analyzed in this study. ORFs deleted en bloc are indicated. (B) The plaque formation and lysogenization capacities of the WT and mutant Sp5 phages are shown. Error bars represent the standard deviations of three independent experiments.