Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Dec 14;6:39048. doi: 10.1038/srep39048

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

PC12 cells transfected with WT hDAT (WT), H547A-hDAT (H547A), H547R-hDAT (H547R), H547D-hDAT (H547D), or Y551H-hDAT (Y551H) were preincubated with or without recombinant Tat_1–86 (rTat_1–86) (140 nM, final concentration) at room temperature for 20 min followed by the addition of [³H]DA. Nonspecific uptake was determined in the presence of 10 μM final concentration of nomifensine. Data are expressed as the ratio of the specific [³H]DA uptake in the presence of Tat to that in the absence of Tat [in DPM: WT hDAT (Tat, 4794 ± 989 vs control, 6858 ± 1393); H547A (Tat, 6190 ± 1474 vs control, 5799 ± 1408); H547R (Tat, 5026 ± 1097 vs control, 4698 ± 1257); H547D (Tat, 2159 ± 557 vs control, 2021 ± 565); and Y551H (Tat, 662 ± 107 vs control, 642 ± 108)] n = 7–8. *p < 0.05 compared with WT hDAT control values.