Table 3. Examples of competition assay results. Specific blocking is demonstrated with bolded numbers.

Blocking VLP/suspension	Case 1, sample taken 4.3.2010			Case 4*, sample taken 11.3.2010			Case 13	Case 16	Case 18
	Antigen in the well			Antigen in the well			Antigen in the well	Antigen in the well	Antigen in the well
	BuV1	BuV2	BuV3	BuV1	BuV2	BuV3	BuV2	BuV3	TuV
none	2.588	2.761	2.896	2.011	0.644	0.174	0.156	0.142	0.314
BuV1	0.030	2.587	2.661	0.127	0.458	0.132	0.177	0.107	0.299
BuV2	2.579	0.038	2.722	1.949	0.167	0.143	0.023	0.111	0.191
BuV3	2.400	2.614	0.080	1.904	0.472	0.151	0.191	0.024	0.377
TuV	2.513	2.720	2.819	1.997	0.525	0.172	0.164	0.116	0.101§
High5 insect cells	2.638	2.845	2.954	2.016	0.074#	0.087#	0.211	0.116	0.401

^*Case 4 is strongly IgG positive for both BuV1 and insect cells (also seen in Table 2). BuV2 and BuV3 VLPs have been shown to have insect cells impurities (data not shown), which causes more reactivity towards BuV2 and BuV3 antigens. This reactivity is completely blocked by insect cell suspension (marked with^#), and the sample is therefore considered to be IgG positive only for BuV1 among the tested viral antigens. The blocking effect of BuV2 VLP towards BuV2 is interpreted to be caused by the insect cell impurities rather that the VLP itself.

^§5x more blocking antigen was used to block the IgG reactivity.