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. 2016 Nov 29;5(11):e396. doi: 10.1038/mtna.2016.101

Figure 4.

Figure 4

Incomplete homology-directed repair (HDR) during the introgression of MSTN p.C313Y mutation. (a) The targeting strategy for introducing the p.C313Y mutation into porcine MSTN locus. The MSTN allele of Piedmontese and Large White are shown. The corresponding amino acid sequences are listed below where the p.C313Y mutation is highlighted in blue. (b) A schematic illustration showing the location of the sgRNA and ssODN. The nucleotide T in blue is responsible for the p.C313Y mutation, while the nucleotide A in red is the silent mutation preventing the recleavage activity on HDR alleles. (c) Genotype and sequencing chromatogram of representative colonies harboring WT allele, incomplete HDR allele or complete HDR allele. The arrowhead indicates the cleavage site of Cas9.