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. 2016 Nov 8;5(11):e383. doi: 10.1038/mtna.2016.86

Figure 2.

Figure 2

miR82-mediated knockdown of Hmgcr in mouse upregulates Ldlr expression. Liver tissue, total liver RNA, and protein lysates were prepared from C57BL/6J mice injected with either miR82 or miRNT. (a) Frozen liver, heart, and kidney sections from mice injected with 50 µg pmiRNT (control) or pmiR82, 48 hours postdelivery. Scale bar = 70 µm. EmGFP signals were only detected in transfected hepatocytes. Blue = DAPI; Green = EGFP. (b) qRT-PCR was performed to detect Hmgcr transcript levels in total liver RNA isolated from mice treated with miR82 or miRNT. (c) Liver lysates were prepared from injected mice and subjected to western blot analysis for the detection of transgene expression. Ldlr protein expression from livers of mice injected with pmiR82, as compared to control pmiRNT. N = 5. Error bars denote SEM. **P < 0.01, ***P < 0.0001 (Student's t-test).