Figure 6.

Ectopic expression of miR-9600 in A549 and SPC-A-1 cells reduces cell migration and invasion motility. (a) A549 and SPC-A-1 cells were loaded onto the top well of a transwell inserts for cell migration or invasion assay. After 24 hours, cells that migrated to the bottom chamber containing serum-supplemented medium were stained with 0.1% crystal violet, visualized under a phase-contrast microscope, and photographed. Bar = 50 μm. (b,c) Total number of cells in five fields was counted manually. (d–h) Expression of MMP-2, MMP-7, and MMP-9 protein in A549 and SPC-A-1 cells after transfection. Assays were performed in triplicate. *P < 0.001, Means ± SEM was shown. Statistical analysis was conducted using student t-test.