Diacylglycerol (DAG) produced by Ipc1 activates Pkc1 through the C1 domain of Pkc1. Activation of Pkc1 maintains the structure of the cell wall, which enables laccase to produce melanin granules deposited in the cell wall. Melanin production is required for pathogenesis of Cn. PI phosphatidylinositol, Ipc1 inositol phosphoryl ceramide synthase 1, Pkc1 protein kinase C 1, l-DOPA-ext l-Dopamine extracellular, l-DOPA-int l-Dopamine intracellular.