Figure 3.

MPK4 Is Required for MYB75-Mediated Anthocyanin Accumulation.

(A) Twelve-day-old Arabidopsis seedlings of mpk4-3, pap1-D, and mpk4-3 pap1-D were grown on plates under low light and moderate high light (high light). Bars = 0.5 cm.

(B) Anthocyanin contents of the seedlings in (A). FW, fresh weight. Error bars represent sd of three replicates (as described in Figure 2B).

(C) Quantitative real-time PCR analysis of DFR, LDOX, and UF3GT transcript levels in 12-d-old mpk4-3, pap1-D, and mpk4-3 pap1-D seedlings grown on plates exposed to low light and moderate high light (high light) for 9 h, respectively. Results were normalized to ACTIN8, and expression levels of the genes in mpk4-3 under low light were set at one unit. Error bars indicate sd of three replicates (as described in Figure 2B).