Figure 6.

Phosphorylation of MYB75 Enhances Its Function in Anthocyanin Accumulation.

(A) Twelve-day-old Arabidopsis seedlings of wild-type, 35S:MYB75^WT/WT, 35S:MYB75^DD/WT, and 35S:MYB75^AA/WT transgenic seedlings grown on plates under low light and moderate high light (high light). Bars = 1 cm.

(B) Anthocyanin contents of the seedlings in (A). FW, fresh weight. Error bars represent sd of three replicates (as described in Figure 2B).

(C) Quantitative real-time PCR analysis showing comparable MYB75 expression levels in 35S:MYB75^WT/WT, 35S:MYB75^DD/WT, and 35S:MYB75^AA/WT transgenic seedlings. Primers were designed to detect both transgenic and endogenous MYB75 transcripts. Results were normalized to ACTIN8, and expression levels of the genes in the 35S:MYB75^WT/WT transgenic seedlings were set at one unit. Error bars indicate sd of three replicates (as described in Figure 2B).

(D) Quantitative real-time PCR analysis of DFR and LDOX transcript levels in 12-d-old wild type, 35S:MYB75^WT/WT, 35S:MYB75^DD/WT, and 35S:MYB75^AA/WT transgenic seedlings grown on plates exposed to low light and moderate high light (high light) for 9 h, respectively. Results were normalized to ACTIN8, and expression levels of the genes in the wild type under low light were set at one unit. Error bars indicate sd of three replicates (as described in Figure 2B).