Figure 10.

B-Dependent Downregulation through the AUG-Stop of NIP5;1 in Animal Systems.

(A) Schematic representation of 5′-NIP5;1(−306):LUC RNA in rabbit. Open boxes represent uORFs.

(B) 5′-NIP5;1(−306):LUC RNA was translated in RRL, and means ± sd of relative LUC activities (n = 3) are shown. Asterisks indicate significant reductions in RNA carrying ⁻¹²²AUGUAA (P < 0.05).

(C) Schematic representation of ProSV40:5′-NIP5;1(−231):LUC RNA. The thick line represents the sequence derived from the NIP5;1 5′-UTR and the thin line represents the 35-nucleotide linker sequence.

(D) Effect of B on reporter activities in transfected HeLa cells. Transfected HeLa cells were incubated under various B conditions, and means ± sd of relative LUC activities (n = 6) are shown. Asterisks indicate significant reductions in +B compared with no supplementation of B (P < 0.05). The “vector 5′-UTR” negative control carries only the vector’s 5′-UTR sequence.