Figure 4.

B-Dependent Reinitiation of NIP5;1.

(A) Schematic representation of 3′-deletion series of Pro35S:5′-NIP5;1(−306):LUC DNA. The thick line represents the sequence corresponding to NIP5;1 mRNA. The thin lines represent the 18- and 15-nucleotide linker sequences at the 5′ and 3′ ends, respectively, of the 5′-UTR.

(B) Effects of spacer deletions on the B response in transfection experiments. Transfected protoplasts were incubated under 500 μM B (+B) or 1 μM B (−B) conditions, and means ± sd of relative LUC activities (n = 8) are shown. Asterisks indicate significant reductions under +B compared with −B conditions (P < 0.05). The “vector 5′-UTR” negative control carries only the vector’s 5′-UTR sequence.