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. 2016 Oct 19;28(11):2830–2849. doi: 10.1105/tpc.16.00481

Figure 5.

Figure 5.

B-Dependent Downregulation Conferred by Introduction of AUG-Stop into MOT2 5′-UTR.

(A) mRNA accumulation in Col-0 roots under 100 µM B (+B) and 0.3 µM B (−B) conditions. Means ± sd of relative mRNA accumulation (n = 3) are shown.

(B) Schematic representation of 5′-MOT2(−333):LUC RNA and the effect of AUGUAA introduction on the B response. RNA carrying 5′-MOT2(−333):LUC with or without AUGUAA was translated in WGE in the presence of various B concentrations. Means ± sd of relative LUC activities (n = 3) are shown. Asterisks indicate significant reduction of reporter activity in RNA having an AUGUAA compared with that in RNA having the original MOT2 5′-UTR sequence (P < 0.05).

(C) and (D) Primer extension (C) and toeprint (D) analyses in WGE with 300 µM B (+B) or without B supplementation (−B). Signals are enlarged under the main image of the AUGUAA lane, and means ± sd of relative intensities are shown (n = 3). Asterisks indicate significant differences (P < 0.05).

(E) Nucleotide sequence around AUG-stop and the positions of PE (magenta) and TP signals (green). Ribosome occupation of RNA having the AUG codon positioned at the P-site is shown.