Figure 6.

DCL4^NLS Produces a Unique Set of siRNAs in Siliques.

(A) Flowchart of sequence analysis pipeline for library size-normalized 20- to 21-nucleotide sRNA reads, uncovering a population of candidate loci that produce sRNAs made by DCL4^NLS. Loci with adjusted P value < 0.05 were considered as candidates.

(B) Heat map showing log(e)-fold differential accumulation of 20- to 21-nucleotide siRNAs in different pairwise library comparisons for a selection of loci.

(C) RNA gel blot detection of siRNAs in siliques of Col (wild type), dcl4 mutant plants, and dcl4 mutants expressing ProUbq10:DCL4^NLS-GFP or ProUbq10:DCL4^∆-GFP, revealing 24-nucleotide species and 21-nucleotide disiRNAs produced by DCL4^NLS (arrow); analysis performed by stripping and reprobing the same membrane.