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. 2015 Jan 30;5:8084. doi: 10.1038/srep08084

Figure 2. The gene expression of the isolated human spermatogonia, pachytene spermatocytes and round spermatids.

Figure 2

(A–B), RT-PCR revealed the transcripts of GPR125, GFRA1, PLZF, UCHL1, RET, SCP1, SCP3, Tesmin, PIWIL2, ACR, TNP1, PRM1 and PRM2 in the freshly isolated human spermatogonia. (C–D), RT-PCR showed the mRNA of SCP1, SCP3, Tesmin, PIWIL2, GPR125, GFRA1, PLZF, UCHL1, RET, ACR, TNP1, PRM1 and PRM2 in the freshly isolated human pachytene spermatocytes. (E–F), RT-PCR displayed the transcripts of ACR, TNP1, PRM1, PRM2, GPR125, GFRA1, PLZF, UCHL1, RET, PIWIL2, SCP1, SCP3 and Tesmin in human round spermatids isolated from human testis tissues. The expression of these genes in human testis tissues served as positive controls. GAPDH served as a loading control of total RNA, and water without DNA was employed as a negative control. The data shown in (A–F) were representatives from eight independent experiments of thirty patients.