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. 2015 Jan 30;5:8084. doi: 10.1038/srep08084

Figure 3. The protein expression of the isolated human spermatogonia, pachytene spermatocytes and round spermatids.

Figure 3

(A–D), Immunocytochemistry revealed the expression of GPR125 (A), GFRA1 (B), UCHL1 (C) and MAGEA4 (D) in the freshly isolated human spermatogonia. Scale bars in A–D = 10 μm. (E), Meiosis spread assays displayed the expression of SCP3 (red fluorescence) and CREST (green fluorescence) in the freshly isolated human pachytene spermatocytes. Scale bar in E = 5 μm. (F), Immunocytochemistry showed the expression of SCP3 in the freshly isolated human pachytene spermatocytes. Scale bar in F = 10 μm. (G–H), Immunocytochemistry revealed the expression of Acrosin (G) and Protamine 2 (H) in the isolated human round spermatids. Scale bars in G and H = 5 μm. The data shown in (A–H) were representatives from eight independent experiments of thirty patients.