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. 2016 Dec 13;18:127. doi: 10.1186/s13058-016-0754-9

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Imaging of see deep brain (SeeDB)-cleared mammary tumours using 2-photon excitation microscopy. Two-photon imaging of SeeDB-cleared human epidermal growth factor receptor 2 (HER2)-positive mammary tumours immunostained with HER2 (green) and 4′,6-diamidino-2-phenylindole (DAPI) nuclear staining (blue) and magnified view (inset). Orthagonal views show XZ (purple line and box) and YZ (red line and box) planes. Thin optical slices (2 μm) and their depth (z value) relative to the first image in the image sequence are also shown. See additional file 18 for a high resolution version of these PDFs and http://rdcu.be/lT3Z for additional high resolution examples of these imaging techniques [38]