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. 2016 Dec 13;9:641. doi: 10.1186/s13071-016-1930-6

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Sb^III susceptibility assay of clonal lines from L. braziliensis (a) and L. infantum (b) non-transfected or transfected with the constructs pIR1BSD or pIR1BSD-EF2. Parasites were incubated in M199 medium in the absence or presence of different concentrations of Sb^III (1.17 to 599.04 μM) for 48 h and the percentage of relative growth was determined using a Z1 Coulter Counter. Mean values ± standard deviations from three independent experiments in triplicate are shown. Statistical analysis was carried out using Student’s t-test. Statistically different values are highlighted as follows: *P < 0.05; **P < 0.01; ***P < 0.001. Pairwise comparisons (a): 1.17 μM: LbWTS vs LbEF2 clone 9 (t₍₉₎ = 6.5, P = 0.0001); LbWTS vs LbEF2 clone 12 (t₍₅₎ = 2.84, P = 0.0361); 2.34 μM: LbWTS vs LbEF2 clone 9 (t₍₇₎ = 6.95, P = 0.0002); LbWTS vs LbEF2 clone 12 (t₍₄₎ = 2.87, P = 0.0455); 4.68 μM: LbWTS vs LbEF2 clone 9 (t₍₅₎ = 6.04, P = 0.0018); LbWTS vs LbEF2 clone 12 (t₍₄₎ = 2.87, P = 0.0453); 9.36 μM: LbWTS vs LbEF2 clone 9 (t₍₇₎ = 22.18, P < 0.0001); LbWTS vs LbEF2 clone 12 (t₍₈₎ = 4.48, P = 0.0021); 18.72 μM: LbWTS vs LbEF2 clone 9 (t₍₁₁₎ = 4.74, P = 0.0006); LbWTS vs. LbEF2 clone 12 (t₍₉₎ = 7.65, P < 0.0001); 37.44 μM: LbWTS vs LbEF2 clone 9 (t₍₅₎ = 2.78, P = 0.0391); LbWTS vs LbEF2 clone 12 (t₍₅₎ = 5.1, P = 0.0038). (b) 18.72 μM: LiWTS vs LiEF2 clone 5 (t₍₉₎ = 2.67, P = 0.0257); 37.44 μM: LiWTS vs LiEF2 clone 5 (t₍₈₎ = 6.14, P = 0.0003); 74.88 μM: LiWTS vs LiEF2 clone 5 (t₍₉₎ = 4.9, P = 0.0008)