Table 1.
EC50 of SbIII, lamivudine, EF2K inhibitor and hydrogen peroxide and corresponding RI for WTS, NDKb- or EF2-overexpressing L. braziliensis lines. EC50 represents effective concentration required to decrease growth by 50%. The values were determined from at least three independent experiments performed in triplicate, using the linear interpolation method [38]
| Parasites | SbIII | Lamivudine | EF2K inhibitor | Hydrogen peroxide | ||||
|---|---|---|---|---|---|---|---|---|
| EC50 (μM) | RI | EC50 (μM) | RI | EC50 (μM) | RI | EC50 (μM) | RI | |
| LbWTS | 7 | – | 766 | – | 173 | – | 213 | – |
| LbpIR1BSD | 8 | – | 733 | – | – | – | 207 | – |
| LbNDKb clone 4 | 12 | 1.7 | 2,110 | 2.8 | – | – | 207 | 1.0 |
| LbNDKb clone 9 | 15 | 2.1 | 2,014 | 2.6 | – | – | 207 | 1.0 |
| LbEF2 clone 9 | 14 | 2.0 | – | – | 211 | 1.2 | – | – |
| LbEF2 clone 12 | 11 | 1.6 | – | – | – | – | – | – |
Chemical formulae and abbreviations: SbIII (potassium antimonyl tartrate); lamivudine (C8H11N3O3S); EF2K inhibitor (C13H15N5O3); hydrogen peroxide (H2O2); WTS wild-type susceptible, Lb L. (V.) braziliensis, pIR1BSD expression vector, NDKb nucleoside diphosphate kinase b, EF2 elongation factor 2, EF2K elongation factor 2 kinase, RI resistance index