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. 2016 Dec 14;6:39105. doi: 10.1038/srep39105

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Copyright © 2016, The Author(s)

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6 week-old female NOD/ShiLtJ mice were i.p.-injected with 200 μg of rat IgG1 or anti-IFN-γ together with 200 μg of anti-PD-L1 every other day for a total of 4 times. (A) Stimulated saliva flow rate normalized to body weight. (B) Real-time PCR analysis of relative AQP5 mRNA levels in the SMG. (C) Immunofluorescence staining of AQP5 protein in SMG sections, and the quantification of the fluorescence intensity of AQP5 staining (right panel). Original magnification: ×400. Data are representative or the average of the analyses of 8 mice for each group.