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. Author manuscript; available in PMC: 2016 Dec 14.
Published in final edited form as: Protein J. 2016 Feb;35(1):1–16. doi: 10.1007/s10930-015-9641-y

Figure 3. Lipid binding and lipid-dependent prohibitin regulation.

Figure 3

A. Western blotting using anti-prohibitin primary antibody demonstrates that prohibitin has different interactions with phospholipids, including cardiolipin and phosphatidylserine. Prohibitin in the retina has high affinity to cardiolipin, while prohibitin in the RPE shows weaker affinity. Microsomal prohibitin in the retina shows high affinity to phosphotidylserine, cardiolipin, and cholesterol. Microsomal fraction applied on the PVDF membrane is considered as a positive control.

B. Cholesterol-dependent prohibitin expression in the RPE. ARPE-19 cells were treated with cholesterol (16 hours). Increased cholesterol levels significantly downregulate PHB1 expression whereas PHB2 levels show less sensitivity toward the elevated cholesterol levels. C. Graphical representation shows that cardiolipin levels decreased in human RPE under elevated oxidative stress.