TABLE 1.
Prohibitin-Binding Proteins. A1-A49 represents prohibitin binding proteins in ARPE19 cells and H1-H48 shows prohibitin binding proteins in human retinal progenitor cells.
| Band | Accession | Protein identified | MOWSE score | %coverage | Mass | PI | Peptide |
|---|---|---|---|---|---|---|---|
| A1 | 83404899 | Kinesin Family member 19 protein | 47325 | 8.2 | 93161 | 6.2 | 7 |
| A1 | 194388398 | Unknown, BAG65583, 110kDa myosin motor domain, ATPase, SH3 | 253758 | 9.0 | 110912 | 8.5 | 7 |
| A6 | 15277503 | ACTB protein partial | 1.59e+7 | 30.0 | 40221 | 5.6 | 9 |
| A13 | 26330300 | Unknown | 633431 | 10.0 | 88790 | 4.7 | 6 |
| A14 | 15277503 | ACTB protein partial | 1.59e+7 | 30.0 | 40221 | 5.6 | 9 |
| A16 | 292352 | Collagen, partial | 189998 | 11.0 | 72107 | 7.3 | 5 |
| A18 | 535012 | NADP+-dependent malic enzyme | 1.06e+6 | 14.9 | 67055 | 7.9 | 6 |
| A18 | 74187742 | Nucleoporin-like protein | 1.95e+6 | 12.0 | 86380 | 6.5 | 6 |
| A22 | 2217933 | PKU-beta (Serine/Threonine kinase) | 2211 | 7.6 | 88883 | 8.9 | 4 |
| A22 | 8392959 | Beta-1,4-galactosyltransferase 2 | 3975 | 15.4 | 41910 | 9.4 | 4 |
| A22 | 113195686 | Lamin-B2 | 3513 | 10.9 | 67319 | 5.4 | 4 |
| A23 | 194388398 | Unknown, BAG65583, 110 kDa myosin motor domain, ATPase, SH3 | 9.80e+7 | 15.1 | 110912 | 8.5 | 12 |
| A25 | 158259341 | PI3Kinase, p85 binding domain protein | 645600 | 11.6 | 124285 | 6.9 | 8 |
| A28 | 40352738 | Ion transport protein | 306309 | 18.5 | 61801 | 5.5 | 5 |
| A32 | 4220898 | Transcriptional co-activator CRSP130 | 441010 | 8.4 | 106209 | 8.5 | 6 |
| A33 | 41945518 | Tiam1 PH domain lipid binding protein | 105719 | 8.6 | 99654 | 8.7 | 7 |
| A34 | 38569540 | 5'-3' exoribonuclease 2 isoform 3 | 140868 | 10.6 | 103802 | 8.2 | 7 |
| A34 | 148705232 | Leucine rich repeats,pyrin death domain | 179814 | 9.4 | 112138 | 8.0 | 8 |
| A35 | 624871 | a1(XIX) collagen chain precursor | 2.94e+7 | 12.9 | 115096 | 8.4 | 8 |
| A41 | 74187742 | Nucleoporin-like protein | 379387 | 10.2 | 86380 | 6.5 | 5 |
| A44 | 62897409 | Beta actin variant | 2.47e+8 | 35.5 | 41723 | 5.3 | 10 |
| A45 | 3603443 | Fibrousheathin I | 192098 | 11.7 | 94617 | 6.0 | 5 |
| A45 | 5834582 | E3 ubiquitin-protein ligase TRIM33 | 66846 | 8.7 | 115982 | 7.1 | 6 |
| A47 | 119582405 | Cadherin tandem repeat,ion binding domain | 4.44e+8 | 18.6 | 88669 | 5.0 | 10 |
| A49 | 13278786 | Nuclear mitotic apparatus protein 1 isoform | 1.85e+7 | 14.2 | 109280 | 8.1 | 11 |
| H1 | 194388398 | Unknown, BAG65583, 110kDa myosin motor domain, ATPase, SH3 | 161675 | 8.6 | 110912 | 8.5 | 7 |
| H3 | 15277503 | ACTB protein, partial | 8.45e+6 | 33.6 | 40221 | 5.6 | 9 |
| H4 | 158256650 | Myosin head motor domain Transmembrane 67, Meckelin | 1.14e+9 | 14.3 | 116422 | 6.2 | 11 |
| H5 | 119603632 | arginine methyltransferase 7, isoform CRA_b | 77142 | 7.3 | 69472 | 5.2 | 4 |
| H5 | 119603470 | Esterase 31, isoform CRA_e | 43420 | 7.7 | 59890 | 5.4 | 4 |
| H6 | 7019942 | 5’-3’ Exonuclease | 6.28e+6 | 17.1 | 63785 | 9.0 | 6 |
| H8 | 56204817 | Actin, alpha 1, skeletal muscle | 1.79e+6 | 30.7 | 32049 | .3 | 8 |
| H11 | 11908000 | BCL-6 corepressor short isoform | 1.78e+7 | 17.8 | 107405 | 8.8 | 9 |
| H11 | 119584312 | Inositol 1,4,5-triphosphate receptor, type 1 | 1.47e+6. | 8.8 | 117553 | 6.2 | 6 |
| H13 | 119612108 | Transmembrane protein 67, isoform CRA_b | 752714 | 10.2 | 109958 | 6.2 | 6 |
| H14 | 5882259 | Genethonin 3 | 9.41e+6 | 15.3 | 89342 | 5.2 | 8 |
| H19 | 14250401 | Actin, beta, partial | 4.89e+6 | 32.3 | 41005 | 5.6 | 9 |
| H21S | 74213524 | ACTB protein, partial | 1.22e+9 | 33.6 | 41751 | 5.3 | 10 |
| H22 | 21758574 | Organic solute transport protein 1 | 63.8 | 20.4 | 38752 | 7.8 | 4 |
| H23 | 14250401 | Actin, beta, partial | 3.70e+7 | 35.9 | 41005 | 5.6 | 10 |
| H23S | 2656123 | p53 binding protein | 2881 | 7.4 | 93501 | 9.6 | 4 |
| H25 | 288541297 | leucine-rich repeat-containing protein 15 isoform b precursor | 1.44e+8 | 27.7 | 64367 | 6.2 | 10 |
| H26 | 2102696 | Karyopherin beta 3 | 6.53e+6 | 13.2 | 123599 | 4.8 | 10 |
| H29 | 115529728 | Guanine nucleotide exchange factor OSTIII | 5.98e+6 | 8.8 | 123524 | 5.7 | 8 |
| H32 | 34189309 | Nuclear Pore Protein 107 | 2.40e+11 | 19.2 | 79971 | 5.2 | 8 |
| H33 | 119597872 | Unknown | 52313 | 8.2 | 112707 | 5.1 | 6 |
| H34 | 272982598 | Anti-HIV-1 gp120 Ig heavy chain | 4.02e+8 | 22.4 | 51810 | 8.1 | 8 |
| H36 | 5305448 | PH domain containing signaling mediator | 175808 | 16.8 | 70047 | 5.7 | 6 |
| H38 | 39644830 | Pleckstrin 2 protein, partial | 152085 | 24.2 | 39711 | 9.5 | 5 |
| H39 | 7661922 | Ras related protein Rab-21 | 38925 | 33.8 | 24348 | 8.1 | 5 |
| H41 | 148692949 | Leucine rich repeat containing 21 | 169639 | 9.6 | 111679 | 6.8 | 5 |
| H44 | 226958677 | Guanine nucleotide exchange factor DSB 3 | 872951 | 7.6 | 123694 | 5.7 | 8 |
| H45 | 148682292 | Retinis pigmentosa 1 homolog (human) | 30379 | 5.1 | 124037 | 9.5 | 6 |
| H48 | 74213524 | Actin like nucleotide binding sugar kinase | 2.37e+7 | 44.3 | 41751 | 5.3 | 12 |