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. 2015 Jul 10;5:10179. doi: 10.1038/srep10179

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(a) Purification of DOI on a HiPrep 16/10 DEAE FF column. (b) Fraction D3 was further purified on a HiPrep 16/10 Phenyl FF (high sub) column. (c) Fraction P1 was further purified on a Superdex 75 10/300 GL column and fraction S1 contained the DOI protein. (d) 15% Native PAGE of DOI (left) and 15% SDS-PAGE of DOI (right). The DOI protein was visualized by silver staining. (e) Size measurement of DOI by mass spectrometry.