Fig. 6.

Broad binds to the stg regulatory locus and overlaps with wing enhancers. (A) An 80 kb window of the stg locus is shown including Broad ChIP-seq signal at 0 h APF (modencode dataset #3806, www.modencode.org), and potential regulatory elements revealed by FAIRE that are accessible during larval stages in the wing and leg disc, but become inaccessible after cell cycle exit in the pharate tissues (McKay and Lieb, 2013). Two regions are shown that overlap with reporters from the Janelia Gal4 collection previously shown to drive expression in the wing (Andrade-Zapata and Baonza, 2014). (B-C) Male animals hemizygous for broad show expression of these reporters in the wing. (D-E) Animals hemizygous for the broad npr3 null allele show loss of Broad expression and ectopic reporter expression in the central wing pouch (for 32B06) and additional expression outside of the anterior-posterior and dorsal-ventral boundary regions (for 32C11). GFP expression is shown in green, and Broad protein expression is shown in magenta.