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. 2016 Oct 17;5(11):1697–1705. doi: 10.1242/bio.021162

Fig. 4.

Fig. 4.

Depletion of ALADIN affects PGRMC2 localisation at the nuclear envelope/perinuclear ER in human skin fibroblasts. NCI-H295R1-TR/AAAS knock-down, NCI-H295R1-TR/Scrambled shRNA, NCI-H295R1-TR cells and skin fibroblasts of a triple A patient (homozygous mutation in Exon 9, c.884G>A, p.Trp295X) and of an anonymised control were stained with mouse anti-ALADIN (green), mouse anti-PGRMC2 (green) and DAPI (blue). Scale bars for anti-ALADIN staining (upper panels): 38 µm (NCI-H295R1-TR/AAAS knock-down), 24 µm (NCI-H295R1-TR/Scrambled shRNA), 32 µm (NCI-H295R1-TR cells) and 38 µm (skin fibroblasts). All scale bars for anti-PGRMC2 staining: 24 µm. NCI-H295R1-TR cells with AAAS knock-down or scrambled shRNA were induced as described previously (Jühlen et al., 2015).