Figure 4. Alteration of the potential landscapes in ESC differentiation.

(A) Averaged Raman spectra of ESCs in +LIF+2i culture (purple) +LIF-2i culture (blue) and -LIF-2i culture for 21 days (red). (B) Plot of scores for PC1 and PC2. Each marker shows the average value of the scores for PC1 and PC2 of the Raman spectra obtained from single nuclei. Error bars show the SDs of the score values from the same nuclei. Spectra were obtained from 62 nucleus for +LIF+2i, 44 for +LIF-2i and 22 for -LIF-2i condition. Data were obtained from three independent cell culture performed on different days. (C) Potential landscapes at the three conditions, which were estimated from U(t) = −ln(P(t)). The contour plots corresponding to the potential landscapes are also shown. The centres of the attractors indicated by the pink and blue pins are at different locations on the PC1-PC2 plane.