Table 1.

PCR conditions

Molecular marker	Primers	Conditions
ITS	AB101	5 min at 94 °C followed by 28 cycles of 1 min at 94 °C, 1 min at 48 °C, 1 min at 72 °C and a last stage of 7 min at 72 °C
	AB102
psbA-trnH	psbA (F)	5 min at 94 °C followed by 28 cycles of 1 min at 94 °C, 1 min at 48 °C, 1 min at 72 °C and a last stage of 7 min at 72 °C
	trnH (R)
rpl16	rpL16F71	5 min at 94 °C followed by 28 cycles of 1 min at 94 °C, 1 min at 48 °C, 1 min at 72 °C and a last stage of 7 min at 72 °C or 5 min at 80 °C; 35 × (95 °C, 1 min; 50 °C, 1 min with a ramp of 0·3 °C s–1; 65 °C, 5 min) and a last stage of 4 min at 65 °C
	rpL16R1516
trnL-rpl32	trnL(UAG)	5 min at 94 °C followed by 28 cycles of 1 min at 94 °C, 1 min at 48 °C, 1 min at 72 °C and a last stage of 7 min at 72 °C
	rpl32
trnQ 5′-rps16	trnQ 5′ MytrnQ Myrps16 rps16	5 min at 94 °C followed by 28 cycles of 1 min at 94 °C, 1 min at 48 °C, 1 min at 72 °C and a last stage of 7 min at 72 °C