Table 1.
Stress | Subject of ANOVA (Data source) | Source of variation | DF | Mean sum of square |
---|---|---|---|---|
— | Callus Induction (CI) (Fig. 1) | Genotype (G) | 2 | 442.476** |
Media (M) | 7 | 2.858** | ||
G × M | 14 | — | ||
Salt induced stress | Viability of callus (VC) (Table 1) | Genotype (G) | 2 | 7422.85** |
Salt concentration (SC) | 4 | 8543.69** | ||
Callus culture period (CCP) | 3 | 193.27** | ||
G × SC | 8 | 581.68** | ||
G × CCP | 6 | 4.36NS | ||
SC × CCP | 12 | 7.27*NS | ||
G × SC × CCP | 24 | 8.48 | ||
Relative growth rate (RGR) (Fig. 3A) | Genotype (G) | 2 | 1.741** | |
Salt concentration (SC) | 4 | 9.414** | ||
G × S | 8 | 0.132 | ||
Tolerance index (TI) (Fig. 3B) | Genotype (G) | 2 | 0.039** | |
Salt concentration (SC) | 4 | 0.453** | ||
G × S | 8 | 0.005 | ||
Relative water content (RWC) (Fig. 3C) | Genotype (G) | 2 | 3.327** | |
Salt concentration (SC) | 4 | 38.644** | ||
G × SC | 8 | 2.119 | ||
Partial air desiccation stress | Plant regeneration (PR) (Table 2) | Genotype (G) | 2 | 604.34** |
Age of callus (AC) | 3 | 803.94** | ||
Partial air desiccation (PAD) | 4 | 686.17** | ||
G × AC | 6 | 18.45NS | ||
G × PAD | 8 | 45.93NS | ||
AC × PAD | 12 | 124.71* | ||
G × AC × PAD | 24 | 35.09 |
* = Significant at P < 0.01, * = Significant at P < 0.05, NS = Non-significant.