FIG. 4.

Chromatin status at the FXN gene is affected by Parnate treatment during reprogramming of FRDA fibroblasts. (A, B) ChIP analyses of FRDA iPSCs (p0) treated with Parnate during the reprogramming protocol (red bars; compare to blue bars of DMSO controls) reveals overrepresentation of the histone H3K4me2 mark in the promoter region and upstream of the GAA repeats, and enrichment in histone H3K4me3 upstream of the GAAs in the FXN locus. No changes in (C) histone H3K9me3 or (D) histone H3K9ac were detected by ChIP at the FXN locus following Parnate treatment. Locations of the primers used in ChIP qPCR are shown in the diagram below the plot (see legend to Fig. 1). *Indicates P < 0.05.