Figure 3.
Efficacy of oncolytic virotherapy and PLD treatment used alone and in combination against i.p. growth of ID8-R in syngeneic mice. (a) C57BL/6 mice (n = 6 –10) were injected i.p. with 2 × 105 ID8-R cells. Oncolytic virotherapy with OVV-Fc (108 PFU delivered i.p.) alone or in combination with PLD (10 mg/kg delivered i.v.) was initiated 10 days later. In parallel experiments, tumor-bearing mice were treated with PLD, or PLD was delivered to tumor bearing mice 8 days before or after virotherapy treatment. Control mice were treated with PBS. (b) Tumor progression was monitored by bioluminescence imaging using the Xenogen IVIS Imaging System. Data points represent mean ± SD. (c) Survival was defined as the point at which mice were killed because of extensive tumor burden. Kaplan–Meier survival plots were prepared and significance was determined using the log-rank method. *P < 0.05, **P < 0.01, ***P < 0.001. OVV, oncolytic vaccinia virus; PLD, pegylated liposomal doxorubicin; PBS, phosphate buffered saline; PFU, plaque forming units; SD, standard deviation.