Figure 5.

Overview of involvement of the MGMT gene in the adrenal with promoter methylation status of the MGMT gene (A), MGMT mRNA (B), and MGMT protein expression (C) in adrenocortical tissues. D, Correlation of MGMT promoter methylation and mRNA expression in ACCs. E, Representative example of MGMT immunohistochemical staining in adrenocortical carcinomas as determined by immunohistochemistry. Antibodies against MGMT stain tumor cell nuclei. Sections were blinded and independently evaluated by two investigators (microscopic magnification, ×40 and ×400). Lines represent medians. ρ represents Spearman's rank correlation coefficient. **, P < .01. NA, normal adrenals; ACA, adrenocortical adenoma; ACC, adrenocortical carcinoma; IRS, Immunoreactivity Score; MGMT, O6-Methylguanine-DNA methyltransferase.