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. 2016 Sep 7;101(12):4564–4573. doi: 10.1210/jc.2016-2531

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This article has been published under the terms of the Creative Commons Attribution License (CC-BY; https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Copyright for this article is retained by the author(s).

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Figure 5. — Transient expression in HEK293 cells and small-scale purification of wild-type and mutated TBL1X. A, SDS-PAGE of the purification of the isolated wild-type TBL1X and mutant WD40 domains. B, SDS-PAGE of the purification of the wild-type and mutant TBL1X-HDAC3-GPS2-SMRT chimera complex. C, Transient expression in HEK293 cells and small-scale purification of wild-type and mutated full-length TBL1X. SDS-PAGE of the purification of the TBL1X, R is the resin, T is the TEV-eluted protein, and P is the peptide-eluted protein. D, CD spectra from 250 to 200 nm at 20°C of wild-type, A366T, and Y458C TBL1X WD40 domains. E, Melting curves at 215 nm from 10°C to 90°C of wild-type, A366T, and Y458C TBL1X WD40 domains.