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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Oral Surg Oral Med Oral Pathol Oral Radiol. 2016 Sep 9;123(1):37–43. doi: 10.1016/j.oooo.2016.08.020

Figure 1.

Figure 1

Culturing of osteocytes from mandibular bone.

A: Enzymatically digested bone explants in DMEM media.

B: Bone explants after 2–3 weeks showing one bone explants (*) and the cells emerging out.

C: FACS graph shows gating of E11 positively labeled cells

D.: Plated E11 positive cells expressing multiple interconnected processes similar to osteocytes (20× magnification)