Fig. 6.

Only one transplanted cell-derived colony-shaped compartment was observed in all evaluated testes. (A) PKH26. (B) DAPI. (C) Dazl (FITC). (D) Merged picture. The circle shows the colony-like cell aggregate of PKH-positive transplanted cells that simultaneously expressed Dazl. Arrows show spermatogonia-like cells that express both PKH26 and the germ cell-specific marker, Dazl. PKH26-labeled BM-MSCs in the seminiferous tubules of recipient testis did not express sertoli cell-specific marker, after 4, 6 and 8 weeks. (E) PKH26 and (F) Vimentin (FITC) (Bars=50 μm).