Figure 4. Proteins at the active site.
a, 5’exon channel formed between the Large and N-terminal domains of Prp8, Cwc21 and Cwc22. b, 5’exon:U5 loop 1 interaction surrounded by Prp8. Th/X denotes Thumb/domain X of Prp8 (residues 1300-1375). c, interactions between the 5’-exon, the N-terminal (purple) and Large (blue) domains of Prp8, and Yju2 (green). Interactions involving protein main and side chains are shown by solid and dotted lines. d, components surrounding U6 Internal Stem-Loop. e, Prp8 and Cef1 (myb1 domain) stabilise the catalytic triplex. HB denotes helix bundle of the RT domain (residues 750-870). f, structure of the catalytic triplex.
