Figure 2.

Fluorescent response of tile-free and tile-associated BiDZ sensors to the presence of different concentrations of D_Mtb (panel A), D_Msg (panel B), R_Mtb (panel C) and R_Msg (panel D); (a) Tile-free BiDZ as shown in Figure 1A; (b) Tile-associated BiDZ, no Hook_D control; (c) Tile-associated BiDZ in the presence of Hook_D as shown in Figure 1B. Reaction mixtures containing BiDZ sensors (see Experimental Section for concentrations of DNA strands) were incubated with different concentrations of correspondent analytes at 55°C. Fluorescent intensities were measured at 517 nm (λ_ex = 485 nm) after 3 hrs of incubation. The data (average values of 3 independent experiments) were used to calculate the LODs listed in Table S2. Linear equations that correspond to each trendline are shown.