Fig. 1.

KSHV ORF57 interacts with hTREX components CHTOP, CIP29 and POLDIP3. Cells were transfected with eGFP or ORF57-eGFP and co-immunoprecipitations performed using GFP-affinity beads. RNase A was added to all whole-cell lysates, unless otherwise stated. Whole-cell lysates (input) and precipitations were analysed by Western blot using GFP-specific and (a) CHTOP-specific, (b) CIP29-specific and (c) POLDIP3-specific antibodies. FL- and CL-ORF57 denotes either full-length (FL) ORF57 or the caspase-7 cleavage product (CL).