Table 2. Mutants used in this study.
| Mutant BAC | Genotype | Primer sequences* |
|---|---|---|
| Merlin-RL13tetO | Wild-type | GCCGCATGTTGCAGACTGAGAAAGAAAGCTTTATTATGAGACATCATACACATAGTATAGaggatgacgacgataagt TCCCCGCCCCATCACCTCGCCTATACTATGTGTATGATGTCTCATAATAAAGCTTTCTTTcaaccaattaaccaattctga |
| Merlin-RL13tetO-UL74stop | UL74stop | AAAAAAGAGATGATAATGGTGAAAGGCATTCCTAAAATTTAACTCCTGATCTCTTGAACGTTCTTGCTCCTTTCCCTaggatgacgacgataagt CCAATACATTACAATTTATGAGGGAAAGGAGCAAGAACGTTCAAGAGATCAGGAGTTAAATTTTAGGAATGCCTTTCAcaaccaattaaccaattctga |
| Merlin-RL13tetO-UL128stop | UL128stop | ACGGCTGAGATTCGCGGGATCGTCACCACCATGACCTAGTCATTGACATGACAGGTCGTACACAACAaggatgacgacgataagt GTAGTTGCAGCTCGTCAGTTTGTTGTGTACGACCTGTCATGTCAATGACTAGGTCATGGTGGTGACGcaaccaattaaccaattctga |
| Merlin-RL13tetO-UL74stop-UL128stop | UL74stop-UL128stop | ACGGCTGAGATTCGCGGGATCGTCACCACCATGACCTAGTCATTGACATGACAGGTCGTACACAACAaggatgacgacgataagt GTAGTTGCAGCTCGTCAGTTTGTTGTGTACGACCTGTCATGTCAATGACTAGGTCATGGTGGTGACGcaaccaattaaccaattctga |
| Merlin-RL13tetO-UL74stop-UL130stop | UL74stop-UL130stop | CTGCCTGCTTCTGTGCGCGGTTTGGGCAACGCCCTGTCTGTAGTCTCCGTGGTCGTAACTAACAGCAAACCAGAATCCaggatgacgacgataagt GTTTAGACCATGGCGGGGACGGATTCTGGTTTGCTGTTAGTTACGACCACGGAGACTACAGACAGGGCGTTGCCCAAAcaaccaattaaccaattctgaPlos |
| Merlin-RL13tetO-UL74stop-UL131stop | UL74stop-UL131stop | GTCTGTTTGTCTGTGCGCCGTGGTGCTGGGTCAGTGCCAGTAGGAAACCGCGGAATAAAACGATTATTACCGAGTACCaggatgacgacgataagt AGCACGCGTCCCAGTAATGCGGTACTCGGTAATAATCGTTTTATTCCGCGGTTTCCTACTGGCACTGACCCAGCACCAcaaccaattaaccaattctga |
| Merlin-RL13tetO-UL74stopREV-UL128stopREV | Revertant | AAAAAAGAGATGATAATGGTGAAAGGCATTCCTAAAATTATGCTCCTGATCTCTATAACGTTCTTGCTCCTTTCCCTaggatgacgacgataagt CCAATACATTACAATTTATGAGGGAAAGGAGCAAGAACGTTATAGAGATCAGGAGCATAATTTTAGGAATGCCTTTCAcaaccaattaaccaattctga ACGGCTGAGATTCGCGGGATCGTCACCACCATGACCCATTCATTGACACGCCAGGTCGTACACAACAaggatgacgacgataagt GTAGTTGCAGCTCGTCAGTTTGTTGTGTACGACCTGGCGTGTCAATGAATGGGTCATGGTGGTGACGcaaccaattaaccaattctga |
*Uppercase letters, HCMV homology; lowercase letters, homology to template plasmid pEP-Kan-S; bold letters, sequence of interest.