Table 2. Bacterial strains, phages and sequencing primers.
| Strain | Comments | Source (reference) | |||||
|---|---|---|---|---|---|---|---|
| Lactococcus lactis ssp. lactis DGCC7271 | Parent | Industrial starter – DuPont Global Culture Collection | |||||
| Lactococcus lactis ssp. lactis DGCC11032 | DGCC7271 pip
mutant |
Industrial starter (this study) | |||||
| Lactococcus lactis ssp. lactis DGCC11785 | DGCC7271 yjaE
mutant |
Industrial starter (this study) | |||||
| Lactococcus lactis ssp. lactis DGCC11572 | DGCC7271 pip yjaE double mutant | This study | |||||
| Lactococcus lactis ssp. lactis IL1403 | bIL67-propagating host | Provided by S. Moineau: GenBank accession no. NC_002662.1 (Bolotin et al., 2001) | |||||
| Phage | Propagation host | Type | Source | Genome (bp) | ORFs | Percentage GC | GenBank accession no. (reference) |
| M5938 | DGCC7271 | c2-type | DuPont collection (isolated 2010, USA) |
22 240 | 38 | 35.9 | KX373687 |
| M5939 | DGCC7271 | c2-type | DuPont collection (isolated 2010, USA) |
nd | – | – | – |
| M5940 | DGCC7271 | c2-type | DuPont collection (isolated 2010, USA) |
nd | – | – | – |
| D4412 | DGCC7271 | c2-type | DuPont collection (isolated 2003, Europe) |
22 884 | 40 | 35.4 | KX373686 |
| M6162 | DGCC11032 | bIL67-type | DuPont collection (isolated 2012, USA) |
21 697 | 38 | 35.8 | KX373688 |
| M6165 | DGCC11032 | bIL67-type | DuPont collection (isolated 2012, USA) |
22 179 | 37 | 35.7 | KX373689 |
| M6202 | DGCC11032 | bIL67-type | DuPont collection (isolated 2012, Canada) |
22 251 | 38 | 35.9 | KX373690 |
| D4410 | DGCC7271 | bIL67-type | DuPont collection (isolated 2003, Europe) |
22 825 | 40 | 35.7 | KX373685 |
| M6653 | DGCC11032 | bIL67-type | Hybrid M5938 :: bIL67 (this study) |
22 157 | 37 | 35.9 | KX373691 |
| M6654 | DGCC11032 | bIL67-type | Hybrid M5938 :: bIL67 (this study) |
22 350 | 38 | 35.9 | KX373692 |
| bIL67 | IL1403 | bIL67-type | Species type | 22 195 | 37 | 36.0 | NC_001629.1 (Schouler et al., 1994) |
| c2 | Not applicable | c2-type | Species type | 22 163 | 39 | 36.3 | NC_001706.1 (Lubbers et al., 1995) |
| Primers | Sequence (5′ – 3′) | ||||||
| pipStartF | TGTCCATTAAAAGGAAGCAGTG | ||||||
| pipEndR | TTTCTTGCACACGTTCTTCG | ||||||
| YjaE F1 | CCATAAAGAAAGGGGAAAAGATG | ||||||
| YjaE F2 | GTGTGCAACAATTATCTGAAGG | ||||||
| YjaE F3 | GAGGTTCAAGTTTAACAAGTGG | ||||||
| YjaE R | TGTGCTTTCTAGGACAAATCC | ||||||
nd, Not determined.