Table 1. LFI results and quantitative B. pseudomallei count of enrichment broth of the experimental soil specimens.

Tube No.	Specimens	B. pseudomallei Inoculation	Enrichment broth on day	LFI test	Quantitative B. pseudomallei count (CFU/ml)	Notes
1	soil 10 g a	105 CFU	0	ND	3.8 x 103	Expected to have 104 CFU/ml
			2	Positive	4.9 x 107
			4	Positive	1.3 x 108	Fungi 1+
			7	Positive	4.5 x 107	Fungi 2+
2	soil 10 g a	104 CFU	0	ND	5.7 x 102	Expected to have 103 CFU/ml
			2	Positive	2.6 x 107
			4	Positive	8.6 x 106	Fungi 1+
			7	Positive	1.2 x 105	Fungus 3+
3	soil 10 g a	103 CFU	0	ND	35	Expected to have 102 CFU/ml
			2	Positive	9.1 x 106
			4	Positive	2.3 x 107	Fungi 1+
			7	Positive	6.1 x 104	Fungus 4+
4	soil 10 g a	102 CFU	0	ND	10	Expected to have 10 CFU/ml
			2	Positive	3.8 x 106
			4	Positive	5.5 x 106	Fungi 1+
			7	Positive	6.4 x 106	Fungi 2+
5	soil 10 g a	10 CFU	0	ND	0	Expected to have 1 CFU/ml
			2	Negative	8.4 x 105
			4	Positive	7.6 x 105	Fungi 1+
			7	Positive	9.0 x 103	Fungi 2+
6	soil 10 g a	10 CFU	0	ND	0	Expected to have 1 CFU/ml
			2	Negative	1.0 x 104
			4	Negative	2.1 x 106
			7	Positive	3.0 x 107	Fungi 1+
7	soil 10 g a	10 CFU	0	ND	0	Expected to have 1 CFU/ml
			2	Negative	2.0 x 104
			4	Negative	1.9 x 106
			7	Positive	1.5 x 107	Fungi 1+
8	soil 10 g a	10 CFU	0	ND	0	Expected to have 1 CFU/ml
			2	Negative	9.0 x 103
			4	Negative	3.0 x 105
			7	Positive	1.8 x 106	Fungi 1+
9	soil 10 g a	10 CFU	0	ND	0	Expected to have 1 CFU/ml
			2	Negative	3.0 x 103
			4	Negative	1.0 x 105	Fungi 1+
			7	Negative	2.0 x 104	Fungi 1+

ND = Not done. Notes show expected quantitative B. pseudomallei count of enrichment broth on day 0, and fungi present on agar plates (1+, 2+, 3+ and 4+ represent the amount of fungi).

^a Soil culture negative for B. pseudomallei was used. Soil were enriched with 10 ml of TBSS-C50 (threonine-basal salt solution plus colistin 50 mg/L) and incubated in air at 40°C for 7 days.