Fig 2. Overexpression of bcl-2 rescues macrophages from etoposide-induced cell death.

Detection of human bcl-2 by intracellular staining in BMDMs derived from CD68(bcl2)tg mice and littermate controls (A). Etoposide-induced cell death in BMDMs derived from transgenic and littermate controls is detected by staining with annexin V and propidium iodide (B). The induction of apoptosis by etoposide in transgenic and littermate BMDMs is further confirmed by detection of activated caspase-3/7 and staining with sytox for detecting membrane permeability (C). Etoposide dose-dependent activation of caspases in BMDMs from littermate and CD68(bcl2)tg mice (D). Staining is representative of at least 3 independent experiments with n = 3–4 per experiment. The mean values are displayed. * denotes P value ≤ 0.05. ** denotes P value ≤ 0.001.