Fig 3. Overexpression of bcl-2 rescues macrophages from bacterial pathogen-induced cell death.

Using a fixable viability dye the percent of dead transgenic or littermate BMDMs was measured after infection with L. pneumophila (A) or S. pyogenes (B). The type of cell death as further characterized by measurement of active caspase-3/7 and cell permeability using the cell event reagent and sytox respectively. Representative staining of transgenic and littermate BMDMs infected with L. pneumophila is shown in (C), and quantified in (D). Representative staining of transgenic and littermate BMDMs infected with S. pyogenes is shown in (E), and quantified in (F). Data shown are representative of at least 3 independent experiments with n = 3–4 per experiment. The mean values are displayed. * denotes P value ≤ 0.05.