Fig 15. Cytokine production from myeloid cells after infection with S. pyogenes.

Production of IL-6 and TNFα was measured by intracellular staining. Grey shaded histograms are uninfected and S. pyogenes infected are depicted with solid black lines. Representative staining of DCs is shown in (A), and the percent of cells expressing cytokines is shown in (B top), and the MFI is shown in (B bottom). Representative staining of macrophages is shown in (C), and the percent of cells expressing cytokines is shown in (D top), and the MFI is shown in (D bottom). Ex vivo isolated macrophages from the spleens of CD68(bcl2)tg and littermate mice infected with S. pyogenes were stained intracellular for TNFα (E). These data are representative of at least 3 independent experiments with n = 3–4 per experiment. The mean values are displayed. * denotes P value ≤ 0.05.