Skip to main content
. 2016 Dec 14;11(12):e0168219. doi: 10.1371/journal.pone.0168219

Fig 1. Protective effect of GB against DOX-induced cardiac cell death.

Fig 1

(A) Time and dose-dependent DOX-induced cardiac cell death was determined by MTT assay. (B) The safety dosage of GB on the cardiomyocytes viability was assessed by MTT assay. (C) Cardiomyocytes were treated with low (LG, 1μM) medium (MG, 5 μM) and high (HG, 50 μM) of GB after DOX treatment for 3 h and no improvement was found on the cardiomyocytes viability in DOX induced cardiotoxicity. (D) Pretreatment with low (LG), medium (MG) and high (HG) doses of GB for 30 min prior to DOX-induced cardiotoxicity increased cardiomyocytes viability. (E) Representative images of the effects of DOX and pretreatment with GB for 30 min on cardiomyocytes proliferation. ****p<0.0001 when compared to control group, #p<0.01, ##p<0.001 when compared to DOX.