Figure 1. Selective labeling of age-defined abDGCs with the optogenetic silencer ArchT using retrovirus.
(A) The schematics of the retroviral vectors (LTR: long terminal repeats; ψ: psi virus packaging sequence). (B) ArchT-GFP fluorescence in labeled abDGCs in the DG of an adult mouse, examined at 60 days post-injection (dpi). (C) ArchT-GFP fluorescence in labeled abDGCs examined at (i) seven dpi, (ii) 14 dpi, (iii) 30 dpi, and (iv) eight months post-injection. (Scale bar, 20 µm; green: ArchT-GFP fluorescence; red: TO-PRO-3 nuclei stain.).