Figure 4. AbDGCs effectively modulated BOLD signals in bilateral hippocampus at seven week maturation.
(A) Opto-fMRI experimental set-up (i) and imaging protocol (ii). (B) No voxels showed changes upon light illumination in the right DG, in GFP-control mice (n = 7 mice). (C) Voxels showed significant BOLD signal changes upon optogenetic silencing of young abDGCs (seven week maturation) in the right DG (n = 8 mice). BOLD signals were aligned to the standard mouse atlas. Color indicates the correlation P value for each voxel. (D) No voxels showed changes upon optogenetic silencing of older abDGCs (16 week maturation, n = 8 mice, same animals as in C). (E) A lager area is affected by optogenetic silencing of the DG in POMC-Arch transgenic mice, including both developmental-born and adult-born DGCs (n = 5 mice). (F) Time courses extracted from the affected voxels of (i) ipsilateral and (ii) contralateral hippocampus of ArchT-GFP group at 7wpi, and (iii) ipsilateral hippocampus of the POMC-Arch group. Green bars indicate the locations of implanted optical fibers.